Hypothesis generation platform
From a gene list to a bench-ready experiment
Most tools stop at differential expression. Prairie ranks your targets, then hands you the reagents, the estimated cost, and the protocol to validate them at the bench.
Prairie BiosciencesValidation plan
From differential expression to a costed, bench-ready validation plan
1. Analyze
DESeq2 differential expression with ML gene prioritization (0-100). No hardcoded gene lists.
Genelog2FCpadjPriority
AURKA+2.43e-892
TOP2A+1.97e-787
MELK+2.11e-684
2. Hypothesize
AURKA is the top-ranked candidate driver.
up 5.3× (log2FC 2.4)high network centralitydruggable
Hypothesis: AURKA knockdown reduces TNBC cell viability.
3. Validate at the bench
A reagent list, an estimated cost, and a protocol, generated with the hypothesis.
ReagentPurposeEst. cost
AURKA qPCR primersconfirm mRNA$45
Anti-AURKA antibodyconfirm protein (WB)$380
AURKA siRNA poolknockdown$420
Alisertib (MLN8237)pharmacologic rescue$185
qPCR + WesternsiRNA knockdownviability ± inhibitor
~$1,030 reagents ~4 weeks go / no-go readout